Night scenery is a new way to showcase the unique charm of classical gardens, and in recent years, related tourism projects have emerged in multiple cities. However, there are still shortcomings in current research on the public perception preferences of night landscapes. The “Zhuozhengwenya” night tour project in Suzhou Humble Administrator’s Garden was selected as the research object. A quality evaluation system was constructed using methods such as SBE, SD and measurement of lighting environment. The quality of the nighttime landscape in Humble Administrator’s Garden was analyzed through a photo collection, field measurements, a questionnaire survey, and SPSS analysis. The results indicate that the public has a high aesthetic tendency towards plots with rich garden elements, a sense of hierarchy, and novel forms; The public’s preference for night landscapes in three types of classical garden spaces is in the order of courtyards, corridors, and interior buildings; The regression model shows that the beauty level is 0.648 times the aesthetic perception factor of the night landscape and 0.520 times the attribute factor of the night landscape lighting. This study constructed an indicator system for evaluating the quality of classical garden night landscapes and obtained a regression model for scenic beauty based on the public perception preferences. Optimization suggestions were proposed for the nighttime landscape quality of Humble Administrator’s Garden, focusing on aspects such as lighting settings and nighttime scene perception, to provide a reference for the development and improvement of other classical garden nighttime landscapes.